|
|
Training Activities
|
subject
|
partner
|
focus
|
teaching
method. |
contact name
|
| Silencing of genes | A.Costanzo R.Iggo |
keratinocytes shRNA lentiviral vectors |
I
|
|
| Overexpression of genes | M.Dobbelstein | Adenovirus vectors |
I
|
|
| Modifications | S.Lain N.La Thangue |
Ubiquitin/SUMO Acetylation |
II
|
|
| Chromatin Immunoprecipitation | I.Simon M.Levrero A.Costanzo G.Blandino |
ChIP on Chip reChIP p63 ChIP p73/63, YAP |
II
|
|
| Localization/Imaging | J.Bartek | GFP-tagged proteins |
III
|
|
| Interaction | Biotecgen G.Del Sal |
E2 tag system Hybrid system |
III
|
|
| Expression analysis | V.Rotter | Array hybridization |
IV
|
|
| Cell cycle | Y.Haupt X.Lu M.Castedo/G.Kroemer |
FACS FACS FACS |
IV
|
|
| Transformation | A.Jochemsen | Focus formation |
V
|
|
| Differentiation | S.Soddu | Muscle cells Hematopoietic cells |
V
|
ciuffini@ifo.it iacovelli@ifo.it |
| 3D cell culture | S.Stromblad | Melanoma |
VI
|
|
| Cell based screens | G.Selivanova | Screening of small molecule libraries |
VI
|
|
| Animal models | C.Marine S.Soddu |
Gene targetting Bone marrow transplantation |
VII
|
chris.marine@dmbr.ugent.be iacovelli@ifo.it |
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